Role of vermicompost and biostimulants in enhancing garlic growth, bulb yield, and quality parameters

Authors

  • Hassan A. HASSAN Cairo University, Faculty of Agriculture, Department of Vegetable Crops, Giza, 12613 (EG)
  • Mohamed M. SHAHEIN Cairo University, Faculty of Agriculture, Department of Vegetable Crops, Giza, 12613 (EG)
  • Mohamed M. EL-MOGY King Faisal University, College of Agricultural and Food Sciences, Department of Arid Land Agriculture, Al-Ahsa, 31982 (SA) https://orcid.org/0000-0001-7598-7557
  • Atef M. SABER Cairo University, Faculty of Agriculture, Department of Vegetable Crops, Giza, 12613 (EG)
  • Suzy M. ABDELAZIZ Horticulture Research Institute, Agricultural Research Centre, Cross Pollinated Vegetable Research Department, 12611 (EG)
  • Omaima S. DARWISH Cairo University, Faculty of Agriculture, Department of Vegetable Crops, Giza, 12613 (EG)

DOI:

https://doi.org/10.15835/nbha53414823

Keywords:

allicin, Allium sativum, bioextracts, bulb quality, morphological properties, nutrient content, organic amendments, sustainable agriculture

Abstract

Organic fertilizers and biostimulants have been widely studied in vegetable crops, but evidence on their individual and combined effects on growth and quality remains limited. Therefore, this study assessed the effectiveness and synergistic potential of selected organic amendments and biostimulants in improving garlic growth, nutrient composition, allicin content, and total yield. Across two growing seasons, garlic plants received the followed treatment: 1) seaweed extract (SW), 2) garlic extract (GE), 3) yeast extract (YE), 4) vermicompost (VC), biochar, and their combinations. Growth traits, nutrient concentrations, allicin content, bulb characteristics, and yield components were measured and compared with an untreated control. All treatments significantly enhanced plant height, leaf number, and leaf area. The co-application of GE + SW along with vermicompost and biochare produced the highest growth performance and nutrient accumulation. Furthermore, this treatment also markedly increased allicin content, improving bulb quality, and resulted in the greatest bulb weight and total yield. Overall, combining organic fertilizers with biostimulants provides a sustainable strategy to enhance garlic productivity and quality while reducing dependence on synthetic inputs. The study recommends the integrated use of GE + SW with vermicompost and biochar as an effective nutrient management approach.

References

Abdelaal K, Attia KA, Niedbała G, Wojciechowski T, Hafez Y, Alamery S, Alateeq TK, Arafa SA (2021). Mitigation of drought damages by exogenous chitosan and yeast extract with modulating the photosynthetic pigments, antioxidant defense system and improving the productivity of garlic plants. Horticulturae 7:510. https://doi.org/10.3390/horticulturae7110510

Ali O, Ramsubhag A, Jayaraman, J (2021). Biostimulant properties of seaweed extracts in plants: Implications towards sustainable crop production. Plants 10(3):531. https://doi.org/10.3390/plants10030531

Allen SF, Grimshaw HF, Rowl AB (1984). Chemical analysis. In Moor PD, Chapman SB (Eds.). Methods in plant ecology. Blackwell. Oxford pp 185‒344.

Arioli T, Villalta ON, Hepworth G, Farnsworth B, Mattner SW (2024). Effect of seaweed extract on avocado root growth, yield and post-harvest quality in far north Queensland, Australia. Journal of Applied Phycology 36(2):745-755. https://doi.org/10.1007/s10811-023-02933-0

Ballabh K, Rana DK, Rawat, SS (2013). Effects of foliar application of micronutrients on growth, yield and quality of onion. Indian Journal of Horticulture 70(2):260-265.

Bose S, Laha B, Banerjee S (2014). Quantification of allicin by high performance liquid chromatography-ultraviolet analysis with effect of post-ultrasonic sound and microwave radiation on fresh garlic cloves. Pharmacognosy Magazine 10(2): 88-93.

Brown JD, Lilliland O (1964). Rapid determination of potassium and sodium in plant materials and soil extracts by flame photometer. In Proceedings of the Society for Horticultural Science 48: 341 - 346.

Di Sario L, Boeri P, Matus JT, Pizzio GA (2025). Plant biostimulants to enhance abiotic stress resilience in crops. International Journal of Molecular Sciences 26(3):1129. https://doi.org/10.3390/ijms26031129

EL-Mogy MM, Adly MA, Shahein MM, Hassan HA, Mahmoud SO, Abdeldaym EA (2024). Integration of biochar with vermicompost and compost improves agro-physiological properties and nutritional quality of greenhouse sweet pepper. Agronomy 14(11):2603. https://doi.org/10.3390/agronomy14112603

EL-Mogy MM, Hassan HA, Aboelghar M, Morsy N, Mahmoud AWM, Abdeldaym EA, Ali AM (2025). Magnesium oxide nanoparticles enhance growth performance, spectral vegetation indices, yield quantity, and quality of green onion under Egyptian conditions. Russian Journal of Plant Physiology 72(4):124. https://doi.org/10.1134/S1021443725601399

Fajdetić NR, Božić Ostojić L, Benković R, Zima D, Blažinkov M, Mirosavljević K, Popović B, Benković-Lačić T (2025). Effects of three organic fertilizers and biostimulants on the morphological traits and secondary metabolite content of lettuce. Horticulturae 11(11):1288. https://doi.org/10.3390/horticulturae11111288

Gao F, Li Q, Wei W, Wang Y, Song W, Yang X, Ji H, Zhou J, Xin Y, Tan Z, Pei J (2024). Preparation of yeast extract from brewer’s yeast waste and its potential application as a medium constituent. Applied Biochemistry and Biotechnology 196(10):6608-6623. https://doi.org/10.1007/s12010-024-04885-8

García-Santiago JC, Lozano Cavazos CJ, González-Fuentes JA, Zermeño-González A, Rascon Alvarado E, Rojas Duarte A, … Alvarado-Camarillo D (2021). Effects of fish-derived protein hydrolysate, animal-based organic fertilisers and irrigation method on the growth and quality of grape tomatoes. Biological Agriculture and Horticulture 37:107-124. https://doi.org/10.1080/01448765.2021.1891458

Gatti N, Maghrebi M, Serio G, Gentile C, Bunea VV, Vigliante I, … Mannino G (2025). Seaweed and yeast extracts as sustainable phytostimulant to boost secondary metabolism of apricot fruits. Frontiers in Plant Science 15:1455156. https://doi.org/10.3389/fpls.2024.1455156

Hamedani SR, Rouphael Y, Colla G, Colantoni A, Cardarelli M (2020). Biostimulants as a tool for improving environmental sustainability of greenhouse vegetable crops. Sustainability 12: 5101. https://doi.org/10.3390/su12125101

Hassan HS, Feleafel MN, El-Lahot MSA, El-Hefny M, Rahman TFA, Mohamed AA, Abd-Elkader DY, Mahdy RM (2024). Biostimulants for enhancing productivity, bioactive components, and the essential oils of garlic with the potential antifungal activity. AMB Express 14(1):130. https://doi.org/10.1186/s13568-024-01790-5

Hatwal PK, Kavita A, Choudhary MK Singh B (2015). Effect of vermicompost, sulphur and micronutrients on yield and quality of garlic (Allium sativum L.) Var.‘G-282’. Annals of Biology 31(1):85-90.

Hayat S, Ahmad H, Ali M, Hayat K, Khan MA, Cheng Z (2018). Aqueous garlic extract as a plant biostimulant enhances growth, antioxidant activities, and disease resistance. Applied Sciences 8(9):1505. https://doi.org/10.3390/app8091505

Hossain ML, Shapna KJ, Li J, Kabir MH, Siddika F, Khandker S, Beierkuhnlein C (2025). Transforming agriculture with vermicompost: 7-year empirical evidence from drought-prone and salinization-affected regions of Bangladesh. Journal of Cleaner Production 508: 145595. https://doi.org/10.1016/j.jclepro.2025.145595

Jędrszczyk E, Kopeć A, Bucki P, Ambroszczyk AM, Skowera B (2019). The enhancing effect of plants growth biostimulants in garlic cultivation on the chemical composition and level of bioactive compounds in the garlic leaves, stems and bulbs. Notulae Botanicae Horti Agrobotanici Cluj-Napoca 47(1): 81-91. https://doi.org/10.15835/nbha47111074

Johnson, R, Joel JM, Puthur, JT (2024). Biostimulants: The futuristic sustainable approach for alleviating crop productivity and abiotic stress tolerance. Journal of Plant Growth Regulation 43(3):659-674. https://doi.org/10.1007/s00344-023-11144-3

Jurado-Flores A, Heredia-Martínez LG, Torres-Cortes G, Díaz-Santos E (2025). Harnessing microalgae and cyanobacteria for sustainable agriculture: Mechanistic insights and applications as biostimulants, biofertilizers and biocontrol agents. Agriculture 15(17):1-31. https://doi.org/10.3390/agriculture15171842

Kenea SA, Abera Goshu T, Chimdessa K (2024). Evaluating the economic feasibility of combined biochar, lime, and inorganic fertilizer rates for garlic (Allium sativum L.) production in Gimbi District, Western Ethiopia. International Journal of Agronomy 2024(1):4551507. https://doi.org/10.1155/2024/4551507

Kumar V, Priya A, Das S, Rahman FH (2025). The effects of different natural formulations on yield and the quality of horticultural crops: a review. Discover Agriculture 3(1):34. https://doi.org/10.1007/s44279-025-00186-8

Li J, Van Gerrewey T, Geelen D (2022). A meta-analysis of biostimulant yield effectiveness in field studies. Frontiers in Plant Sciences 11:350. https://doi.org/10.3389/fpls.2022.836702

Manoj Kumar MK, Khushboo Kathayat KK, Singh SK, Lovepreet Singh LS, Tejinder Singh TS (2018). Influence of bio-fertilizers application on growth, yield and quality attributes of cucumber (Cucumis sativus L.): A review. Plant Archives 18(2):2329-2334

Manzoor A, Naveed MS, Ali RM, Naseer MA, Ul-Hussan M, Saqib M, Hussain S, Farooq M (2024). Vermicompost: A potential organic fertilizer for sustainable vegetable cultivation. Scientia Horticulturae 336:113443. https://doi.org/10.1016/j.scienta.2024.113443

Mubarak A, Hassan HA, Ismail AM, EL-Mogy MM, Gaafer MA, Abdeldaym EA (2025). Effect of different phosphorus fertilizers sources on growth, productivity, and bulb quality of onion plants. Journal of Global Innovations in Agricultural Sciences. 13(4):1337-1344. https://doi.org/10.22194/JGIAS/25.1758

Osman HS, Gao Y, Luo Z, Alharbi K, Rashwan E, Omara AE, Hafez EM (2025). Integrative use of biochar and biostimulants improves cadmium detoxification and yield in cotton. Science of The Total Environment 981:179585. https://doi.org/10.1016/j.scitotenv.2025.179585

Pączka G, Mazur-Pączka A, Garczyńska M, Kostecka J, Butt KR (2021). Garlic (Allium sativum L.) cultivation using vermicompost-amended soil as an aspect of sustainable plant production. Sustainability 13(24):13557. https://doi.org/10.3390/su132413557

Parađiković N, Teklić, T, Zeljković, S, Lisjak, M, Špoljarević M. (2019). Biostimulants research in some horticultural plant species - A review. Food and Energy Security 8(2):00162. https://doi.org/10.1002/fes3.162

Petropoulos SA, Fernandes Â, Plexida S, Chrysargyris A, Tzortzakis N, Barreira JCM, Barros L, Ferreira ICFR (2020). Biostimulants application alleviates water stress effects on yield and chemical composition of greenhouse green bean (Phaseolus vulgaris L.) Agronomy 10:181. https://doi.org/10.3390/agronomy10020181

Rady H, Nashwa I (2018). Quality improvement and seed yield of two garlic cultivars (Allium sativam L.) by seaweed extract and mycorrhizae. Alexandria Journal of Agricultural Sciences 63(1):41-51.

Rady MM, Salama MM, Kuşvuran S, Kuşvuran A, Ahmed AF, Ali EF, Farouk HA, Osman AS, Selim KA, Mahmoud AE (2023). Exploring the role of novel biostimulators in suppressing oxidative stress and reinforcing the antioxidant defense systems in Cucurbita pepo plants exposed to cadmium and lead toxicity. Agronomy 13:1916. https://doi.org/10.3390/agronomy13071916

Rana A, Singh S, Bakshi M, Singh SK (2020).Studied on genetic variability, correlation and path analysis for morphological, yield and yield attributed traits in okra [Abelmoschus esculentus (L.) Monech]. Internationa Journal of Agricultural and Statistical Sciences 6(1):387-94.

Rohith MS, Sharma R, Singh SK (2021). Integration of panchagavya, neemcake and vermicompost improves the quality of chilli production. Journal of Applied Horticulture 23(2):212-218. https://doi.org/10.37855/jah.2021.v23i02.39

Sánchez AS, Juárez M, Sánchez-Andreu J, Jordá J, Bermúdez D (2005). Use of humic substances and amino acids to enhance iron availability for tomato plants from applications of the chelate FeEDDHA. Journal of Plant Nutrition 28:1877-1886. https://doi.org/10.1080/01904160500306359

Sarkar S, Naik SK, Dutta A, Purakayastha TJ, Mali SS (2025). Vermicompost enhances soil fertility and brinjal yield in acidic alfisol. International Journal of Vegetable Science 9:1-25. https://doi.org/10.1080/19315260.2025.2555552

Shahrajabian MH, Chaski C, Polyzos N, Petropoulos SA (2021). Biostimulants application: a low input cropping management tool for sustainable farming of vegetables. Biomolecules 11(5):698. https://doi.org/10.3390/biom11050698

Shang XC, Zhang M, Zhang Y, Li Y, Hou X, Yang L (2023). Combinations of waste seaweed liquid fertilizer and biochar on tomato (Solanum lycopersicum L.) seedling growth in an acid-affected soil of Jiaodong Peninsula, China. Ecotoxicology and Environmental Safety 260:115075. https://doi.org/10.1016/j.ecoenv.2023.115075

Shukla PS, Prithiviraj B (2021). Ascophyllum nodosum biostimulant improves the growth of Zea mays grown under phosphorus impoverished conditions. Frontiers in Plant Science 11:1-17. https://doi.org/10.3389/fpls.2020.601843

Singh A, Sharma K, Chahal HS, Kaur H, Hasanain M (2025). Seaweed-derived plant boosters: revolutionizing sustainable agriculture. Frontiers in Soil Science 5:1504045. https://doi.org/10.3389/fsoil.2025.1504045

Staykov N, Kanojia A, Lyall R, Ivanova V, Alseekh S, Petrov V, Gechev T (2025). Sustainable agriculture through seaweed biostimulants: a two-year study demonstrates yield enhancement in pepper and eggplant. Frontiers in Plant Science 16:1655340. https://doi.org/10.3389/fpls.2025.1655340

Szczepanek M, Wilczewski E, Pobereżny J, Wszelaczyńska E, Ochmian I (2017). Carrot root size distribution in response to biostimulant application. Acta Agriculturae Scandinavica, Section B - Soil & Plant Science 67(4):334-339. https://doi.org/10.1080/09064710.2017.1278783

Szparaga A, Kuboń M, Kocira S, Czerwińska E, Pawłowska A, Hara P, Kobus Z, Kwaśniewski D (2019). Towards sustainable agriculture - agronomic and economic effects of biostimulant use in common bean cultivation. Sustainability 11:4575. https://doi.org/10.3390/su11174575

Thirumalesh S, Singh SK, Upadhyay P, Bakshi M (2025). Seaweed extract: a potential modulator of productivity and bulb quality in onion (Allium cepa). The Nucleus. 3:1-9. https://doi.org/10.1007/s13237-025-00603-9

Verma P, Singh G, Singh SK., Bakshi M, Mirza AA, Mehandi S, Vijayvargiya V (2025). Correlation, path-coefficient and principal component analysis association among quantitative traits in strawberry to unlock potential of vertical farming system. Kuwait Journal of Science 52(1):100303. https://doi.org/10.1016/j.kjs.2024.100303

Wang Y, Deng C, Cota-Ruiz K, Peralta-Videa JR, Sun Y, Rawat S, … Gardea-Torresdey JL (2020). Improvement of nutrient elements and allicin content in green onion (Allium fistulosum) plants exposed to CuO nanoparticles. Science of the Total Environment 725:138387. https://doi.org/10.1016/j.scitotenv.2020.138387

Yakob BK, Mam EL, Sabirovich GM (2024). Role of biostimulants on the advancement of vegetable production: A review. Agricultural Science and Technology 16(4):3-17. https://doi.org/10.15547/ast.2024.04.035

Zhang Q, Liu J, Jeong SJ, Masabni J, Niu G (2025). Biostimulants applied in seedling stage can improve onion early bulb growth: cultivar- and fertilizer-type-specific positive effects. Horticulturae 11:402. https://doi.org/10.3390/horticulturae11040402

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Published

2025-12-19

How to Cite

HASSAN, H. A., SHAHEIN, M. M., EL-MOGY, M. M., SABER, A. M., ABDELAZIZ, S. M., & DARWISH, O. S. (2025). Role of vermicompost and biostimulants in enhancing garlic growth, bulb yield, and quality parameters. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 53(4), 14823. https://doi.org/10.15835/nbha53414823

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Research Articles
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DOI: 10.15835/nbha53414823

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