Effects of preharvest melatonin and boric acid applications on the pomological and biochemical characteristics of highbush blueberry (Vaccinium corymbosum cv. ‘Camellia’)
DOI:
https://doi.org/10.15835/nbha53414837Keywords:
antioxidant activity, blueberry, preharvest foliar application, nutritional properties, sustainable agricultureAbstract
The application of boric acid and melatonin in blueberry cultivation has recently gained attention for their potential to enhance fruit quality and biochemical stability. Boric acid and melatonin contribute to cell wall integrity, pollen tube development, and nutrient transport, whereas melatonin, a bioactive compound with strong antioxidant properties, improves stress tolerance and supports fruit quality. In this study, the synergistic effects of foliar applications of boric acid (0, 5, 10, 15 ppm), melatonin (0, 5, 10, 15 ppm), and some combinations (0, 2.5+2.5, 5+5, 7.5+7.5 ppm) at early bloom, full bloom, and post-bloom stages were investigated on the pomological and biochemical traits of highbush blueberry (Vaccinium corymbosum cv. ‘Camellia’). The combined treatment of 5+5 ppm melatonin + boric acid produced the highest ascorbic acid content, while the 7.5+7.5 ppm combination significantly enhanced antioxidant activity. Boric acid alone at 5 and 10 ppm also improved antioxidant capacity. Although no significant differences were observed in fruit size or firmness, the treatments notably influenced titratable acidity, anthocyanin accumulation, and ascorbic acid content. These findings indicate that preharvest melatonin and boric acid applications can modulate key biochemical attributes, such as ascorbic acid, anthocyanins, and antioxidant activity, thereby improving the nutritional and functional quality of highbush blueberry fruits.
References
Ahmad S, Muhammad I, Wang GY, Zeeshan M, Yang L, Ali I, Zhou XB (2021). Ameliorative effect of MT improves drought tolerance by regulating growth, photosynthetic traits and leaf ultrastructure of maize seedlings. BMC Plant Biology 21(1):368. https://doi.org/10.1186/s12870-021-03160-w
Al-Huqail AA, Khan MN, Ali HM, Siddiqui MH, Al-Huqail AA, AlZuaibr FM, … Al-Humaid LA (2020). Exogenous MT mitigates boron toxicity in wheat. Ecotoxicology and Environmental Safety 201:110822. https://doi.org/10.1016/j.ecoenv.2020.110822
Arnao MB, Hernández-Ruiz J (2018) MT and its relationship to plant hormones. Annals of Botany 121(2):195-207. https://doi.org/10.1093/aob/mcx114
Arunkumar BR, Thippeshappa GN, Anjali MC, Prashanth KM (2018). Boron: A critical micronutrient for crop growth and productivity. Journal of Pharmacognosy and Phytochemistry 7(2):2738-2741.
Bal E (2021). Effect of melatonin treatments on biochemical quality and postharvest life of nectarines. Journal of Food Measurement and Characterization 15(1):288-295. https://doi.org/10.1007/s11694-020-00636-5
Balbontín C, Ayala H, M Bastías R, Tapia G, Ellena M, Torres C, … Silva H (2013). Cracking in sweet cherries: A comprehensive review from a physiological, molecular, and genomic perspective. Chilean Journal of Agricultural Research 73(1):66-72. http://dx.doi.org/10.4067/S0718-58392013000100010
Bor JY, Chen HY, Yen GC (2006). Evaluation of antioxidant activity and inhibitory effect on nitric oxide production of some common vegetables. Journal of Agricultural and Food Chemistry 54(5):1680-1686. https://doi.org/10.1021/jf0527448
Bozhuyuk MR, Ercisli S, Ayed RB, Jurikova T, Fidan H, Ilhan G, … Sagbas HI (2020). Compositional diversity in fruits of rowanberry (Sorbus aucuparia L.) genotypes originating from seeds. Genetika 52(1):55-65. https://doi.org/10.2298/GENSR2001055B
Carrión-Antolí A, Martínez-Romero D, Guillén F, Zapata PJ, Serrano M, Valero D (2022). Melatonin pre-harvest treatments leads to maintenance of sweet cherry quality during storage by increasing antioxidant systems. Frontiers in Plant Science 13:863467. https://doi.org/10.3389/fpls.2022.863467
Chang CC, Yang MH, Wen HM, Chern JC (2002). Estimation of total flavonoid content in propolis by two complementary colorimetric methods. Journal of Food and Drug Analysis 10(3):3. https://doi.org/10.38212/2224-6614.2748
Dou J, Wang J, Tang Z, Yu J, Wu Y, Liu Z, … Tian Q (2022). Application of exogenous melatonin improves tomato fruit quality by promoting the accumulation of primary and secondary metabolites. Foods 11(24):4097. https://doi.org/10.3390/foods11244097
Fernández V, Sotiropoulos T, Brown PH (2013). Foliar fertilization: Scientific principles and field practices (1st ed). International Fertilizer Industry Association (IFA). Paris, France. https://www.fertilizer.org/resource/foliar-fertilization-scientific-principles-and-field-practices/
García-Sánchez F, Simón-Grao S, Martínez-Nicolás JJ, Alfosea-Simón M, Liu C, Chatzissavvidis C, … Cámara-Zapata JM (2020). Multiple stresses occurring with boron toxicity and deficiency in plants. Journal of Hazardous Materials 5(397):122713. https://doi.org/10.1016/j.jhazmat.2020.122713
Giusti MM, Wrolstad RE (2003). Acylated anthocyanins from edible sources and their applications in food systems. Biochemical Engineering Journal 14(3):217-225. https://doi.org/10.1016/S1369-703X(02)00221-8
Hu W, Yang H, Tie W, Yan Y, Ding Z, Liu Y, … Jin Z (2017). Natural variation in banana varieties highlights the role of melatonin in postharvest ripening and quality. Journal of Agricultural and Food Chemistry 65:9987-9904. https://doi.org/10.1021/acs.jafc.7b03354
Hussain M, Ali Q, Nasir F (2020). Synergistic effects of boron and melatonin in improving plant tolerance to abiotic stress. Journal of Plant Nutrition and Soil Science 183:(4):587-597. https://doi.org/10.1002/jpln.201900607
Jannatizadeh A (2019). Exogenous melatonin applying confers chilling tolerance in pomegranate fruit during cold storage. Scientia Horticulturae 27(246):544-549. https://doi.org/10.1016/j.scienta.2018.11.027
Khalaj K, Ahmadi N, Souri MK (2017). Improvement of postharvest quality of asian pear fruits by foliar application of boron and calcium. Horticulturae 3(1):15. https://doi.org/10.3390/horticulturae3010015
Kumari A, Singh SK, Mathpal B, Verma KK, Garg VK, Bhattacharyya M, Bhatt R (2023). The biosynthesis, mechanism of action, and physiological functions of melatonin in horticultural plants: A review. Horticulturae 9(8):913. https://doi.org/10.3390/horticulturae9080913
Li C, Feng J, Huang WY, An XT (2013). Composition of polyphenols and antioxidant activity of rabbiteye blueberry (Vaccinium ashei) in Nanjing. Journal of Agricultural and Food Chemistry 61(3):523-31. https://doi.org/10.1021/jf3046158
Liu C, Zheng H, Sheng K, Liu W, Zheng L (2018). Effects of melatonin treatment on the postharvest quality of strawberry fruit. Postharvest Biology and Technology 139:47-55. https://doi.org/10.1016/j.postharvbio.2018.01.016
Liu S, Huang H, Huber DJ, Pan Y, Shi X, Zhang Z (2020). Delay of ripening and softening in ‘Guifei’ mango fruit by postharvest application of melatonin. Postharvest Biology and Technology 163:111136. https://doi.org/10.1016/j.postharvbio.2020.111136
Liu Y, Zhang M, Zhang Z (2021). The role of melatonin in enhancing plant growth and stress tolerance. Frontiers in Plant Science 12:697315. https://doi.org/10.3389/fpls.2021.697315
Lorente-Mento JM, Guillén F, Castillo S, Martínez-Romero D, Valverde JM, Valero D, Serrano M (2021). Melatonin treatment to pomegranate trees enhances fruit bioactive compounds and quality traits at harvest and during postharvest storage. Antioxidants 10(6):820. https://doi.org/10.3390/antiox10060820
Mansouri S, Koushesh Saba M, Sarikhani H (2023). Exogenous melatonin delays strawberry fruit ripening by suppressing endogenous ABA signaling. Scientific Reports 13:14209. https://doi.org/10.1038/s41598-023-41311-1
Moussa HR, Algamal SM (2017). Does exogenous application of melatonin ameliorate boron toxicity in spinach plants?. International Journal of Vegetable Science 23(3):233-245. https://doi.org/10.1080/19315260.2016.1243184
Okatan V, Aşkın MA, Polat M, Bulduk I, Çolak AM, Güçlü SF, ... Caruso G (2023). Effects of melatonin dose on fruit yield, quality, and antioxidants of strawberry cultivars grown in different crop systems. Agriculture 13(1):71. https://doi.org/10.3390/agriculture13010071
Pereira GL, Siqueira JA, Batista-Silva W, Cardoso FB, Nunes-Nesi A, Araújo WL (2021). Boron: More than an essential element for land plants? Frontiers in Plant Science 14(11):610307. https://doi.org/10.3389/fpls.2020.610307
Qu G, Wu W, Ba L, Ma C, Ji N, Cao S (2022). MT enhances the postharvest disease resistance of blueberries fruit by modulating the jasmonic acid signaling pathway and phenylpropanoid metabolites. Frontiers in Chemistry 22(10):957581.https://doi.org/10.3389/fchem.2022.957581
Retamales JB, Hancock JF (2018). Blueberries: Crop production science in horticulture (2nd ed.). CABI. Boston, MA, USA.
Rivera S, Giongo L, Cappai F, Kerckhoffs H, Sofkova-Bobcheva S, Hutchins D, East A (2022). Blueberry firmness-a review of the textural and mechanical properties used in quality evaluations. Postharvest Biology and Technology 192:112016. https://doi.org/10.1016/j.postharvbio.2022.112016
Routray W, Orsat V (2011). Blueberries and their anthocyanins: factors affecting biosynthesis and properties. Comprehensive Reviews in Food Science and Food Safety 10(6):303-320. https://10.1111/j.1541-4337.2011.00164.x
Shang F, Liu R, Wu W, Han Y, Fang X, Chen H, Gao H (2021). Effects of melatonin on the components, quality and antioxidant activities of blueberry fruits. LWT – Food Science and Technology 147:111582. https://doi.org/10.1016/j.lwt.2021.111582
Sharafi Y, Aghdam MS, Luo Z, Jannatizadeh A, Razavi F, Fard JR, Farmani B (2019). Melatonin treatment promotes endoge-nous melatonin accumulation and triggers GABA shunt pathway activity in tomato fruits during cold storage. Scientia Horticulturae 254:222-227. https://doi.org/10.1016/j.scienta.2019.04.056
Shi C, Zhang J, Jia Z, Yang X, Zhou Z (2021). Intelligent pH indicator films containing anthocyanins extracted from blueberry peel for monitoring tilapia fillet freshness. Journal of the Science of Food and Agriculture 101(5):1800-1811. https://doi.org/10.1002/jsfa.10794
Shireen F, Nawaz MA, Chen C, Zhang Q, Zheng Z, Sohail H, … Bie Z (2018). Boron: functions and approaches to enhance its availability in plants for sustainable agriculture. International Journal of Molecular Sciences 19(7):1856. https://doi.org/10.3390/ijms19071856
Sider J, Smith A, Lee T (2022). Effects of boron and melatonin on plant growth and stress tolerance. Journal of Agricultural Science 58(3):124-138. https://doi.org/10.1016/j.jags.2022.01.023
Silva S, Costa EM, Veiga M, Morais RM, Calhau C, Pintado M (2020). Health promoting properties of blueberries: A review. Critical Reviews in Food Science and Nutrition 60(2):181-200. https://doi.org/10.1080/10408398.2018.1518895
Singleton VL, Rossi JA (1965). Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. American Journal of Enology and Viticulture 16(3):144-58. https://doi.org/10.5344/ajev.1965.16.3.144
Sun Q, Zhang N, Wang J, Zhang H, Li D, Shi J, Zhao B … Guo YD (2014). Melatonin promotes ripening and improves quality of tomato fruit during postharvest life. Journal of Experimental Botany 66(3):657-668. https://doi.org/10.1093/jxb/eru332
Takano J, Tanaka M (2023). Boron‐sensing mechanisms involved in boron transport regulation in plants. Journal of Plant Nutrition and Soil Science 1-8. https://doi.org/10.1002/jpln.202300029
Verde A, Míguez JM, Gallardo M (2022). Role of MT in apple fruit during growth and ripening: possible interaction with ethylene. Plants 11(5):688. https://doi.org/10.3390/plants11050688
Wang LJ, Wu J, Wang HX, Li, SS, Zheng XC, Du H, … Wang LS (2015). Composition of phenolic compounds and antioxidant activity in the leaves of blueberry cultivars. Journal of Functional Foods 16:295-304. https://doi.org/10.1016/j.jff.2015.04.027
Wenfei J, Xiaoqiong W, Jingheng M, Lixin W, Linyu L, Jinying L, … Lin W (2023). Exogenous application of melatonin improves the growth and physiological properties of blueberry seedlings under salt stress. Biotechnology & Biotechnological Equipment 37(1):2202781. https://doi.org/10.1080/13102818.2023.2202781
Xu T, Chen Y, Kang H (2019). Melatonin is a potential target for improving post-harvest preservation of fruits and vegetables. Frontiers in Plant Science 10:1388. https://doi.org/10.3389/fpls.2019.01388
Yang W, Yang H, Ling L Chun C, Peng L (2023). Tolerance and physiological responses of Citrus rootstock cultivars to boron toxicity. Horticulturae 9(1):44. https://doi.org/10.3390/horticulturae9010044
Yousef EAA, Nasef I (2023). Effect of boron foliar application and different fruit-set orders on summer squash seed yield and quality. Egyptian Journal of Horticulture 50(1):85-95. https://doi.org/10.21608/ejoh.2023.167396.1216
Zhang L, Liu Z, Wang Y (2020). Effects of boron and melatonin on plant growth and stress resistance. Plant Growth Regulation 92(2):241-252. https://doi.org/10.1007/s10725-020-00523-6
Zhang XC, Zhu YQ, Wang YN Luo C, Wang X (2017). Effects of different plant growth regulators on blueberry fruit quality. In: IOP Conference Series: Earth and Environmental Science. IOP Publishing p 012038. https://doi.org/10.1088/1755-1315/81/1/012038
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Copyright (c) 2025 Volkan OKATAN, Nafiye UNAL, Pinar KALKAN, Melike URFALI, Huseyin KALKAN, Tugce SELVI, Hanifeh Seyed HAJIZADEH, Halil Ibrahim SAGBAS
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